Isolation of DNA from plant cells by gel
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P.F. Lurquin; G. Tshitenge; G. Delaunoit; L. Ledoux
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Article
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1975
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Elsevier Science
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English
β 568 KB
A method is described which allows the obtention of total DNA from cells of Chlamydomonas reinhardi in 1 day. It is based on the gel filtration on agarose of crude lysates incubated for 1 hr with pronase and subsequently brought to 2 M NaCl in order to dissociate nucleoprotein complexes. The DNA ob