Improvement of the extraction of penicil
โ
Isabel S. Novella; Claire Fargues; Georges GrรฉVillot
๐
Article
๐
1994
๐
John Wiley and Sons
๐
English
โ 396 KB
๐ 2 views
Several techniques for protein extraction were tested for recovering penicillin acylase from a recombinant strain of Escherichia coli. These techniques include chemical [guanidine hydrochloride, Triton X-100, ethylenediaminetetraacetic acid (EDTA), ethanol/toluene], physical (sonication, freeze-and-