Large-Scale isolation of acid phosphatas
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R. A. Fisher; H. Harris; M. Houldsworth; M. D. Lilly; P. Dunnill
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Article
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1968
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John Wiley and Sons
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English
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A laboratory process for the isolation of type B acid phosphatase from human erythrocytes has been scaled up 80 times. The enzyme was purified 1300-fold by adsorption on calcium phosphate gel, ammonium sulfate precipitation, and gel filtration. The yield was 277,. Results for the type A enzyme are a