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Isolation of three acid phosphatase isozymes from human erythrocytes

✍ Scribed by Marilyn R. Fenton; K.E. Richardson


Book ID
118845618
Publisher
Elsevier Science
Year
1967
Tongue
English
Weight
507 KB
Volume
120
Category
Article
ISSN
0003-9861

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Large-Scale isolation of acid phosphatas
✍ R. A. Fisher; H. Harris; M. Houldsworth; M. D. Lilly; P. Dunnill πŸ“‚ Article πŸ“… 1968 πŸ› John Wiley and Sons 🌐 English βš– 691 KB

A laboratory process for the isolation of type B acid phosphatase from human erythrocytes has been scaled up 80 times. The enzyme was purified 1300-fold by adsorption on calcium phosphate gel, ammonium sulfate precipitation, and gel filtration. The yield was 277,. Results for the type A enzyme are a