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Isolation of the structural gene for alcohol dehydrogenase by genetic complementation in yeast

✍ Scribed by Williamson, Valerie M.; Bennetzen, Jeffrey; Young, Elton T.; Nasmyth, Kim; Hall, Benjamin D.


Book ID
109717277
Publisher
Nature Publishing Group
Year
1980
Tongue
English
Weight
429 KB
Volume
283
Category
Article
ISSN
0028-0836

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Both insect and mammalian genes have previously been cloned by genetic complementation in yeast. In the present report, we show that the method can be applied also to plants. Thus, we have cloned a rape cDNA for 3-isopropylmalate dehydrogenase (IMDH) by complementation of a yeast leu2 mutation. The