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Isolation of the genomic clone for pathogenesis-related protein 1a fromNicotiana tabacumcv. Xanthi-nc

✍ Scribed by George Payne; T. Dawn Parks; William Burkhart; Sandra Dincher; Patricia Ahl; Jean Pierre Metraux; John Ryals

Publisher: Springer
Year: 1988
Tongue: English
Weight: 457 KB
Volume: 11
Category: Article
ISSN: 0167-4412
DOI: 10.1007/bf00015662

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✦ Synopsis

We describe the isolation of the chromosomal gene for pathogenesis-related protein la from Nicotiana tabacure. A 2 kb fragment containing the PR-la gene as well as 5' and 3' flanking DNA has been sequenced and the transcriptional start site has been determined by primer extension and S1 nuclease mapping. 80070 of the protein sequence from purified PR-la and 20% of the sequence of purified PR-lb has also been determined and used to verify the nomenclature established for the PR-1 cDNAs.

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✍ George Payne; Wayne Middlesteadt; Nalini Desai; Shericca Williams; Sandra Dinche 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 115 KB

Complementary DNA clones encoding the PR-1 proteins have been isolated and protein sequence has been determined from the purified proteins to allow a correlation of the cDNAs and the proteins . The PR-1 cDNAs from tobacco share 90~o nucleotide and amino acid homology with one another . Cornelissen e