Isolation and sequence of a genomic clone encoding the basic form of pathogenesis-related protein 1 fromNicotiana tabacum

✍ Scribed by George Payne; Wayne Middlesteadt; Nalini Desai; Shericca Williams; Sandra Dincher; Michael Carnes; John Ryals

• Publisher: Springer
• Year: 1989
• Tongue: English
• Weight: 115 KB
• Volume: 12
• Category: Article
• ISSN: 0167-4412
• DOI: 10.1007/bf00036973

✦ Synopsis

Complementary DNA clones encoding the PR-1 proteins have been isolated and protein sequence has been determined from the purified proteins to allow a correlation of the cDNAs and the proteins . The PR-1 cDNAs from tobacco share 90~o nucleotide and amino acid homology with one another . Cornelissen etal. have recently reported the isolation of a TMV infectioninduced cDNA, cluster G, from tobacco, that has 65 ~o homology to the PR-lb cDNA. The deduced cluster G protein has more basic residues than the PR-1 proteins and so may be a basic isoform of PR-1. Here (Fig. ) we report the isolation and nucleotide sequence of the tobacco gene that encodes the cluster G cDNA. This gene is 64~o homologous to the PR-la gene within the coding sequence for the mature protein. The homology is not significant through either the signal peptide coding region or the 3' untranslated region. There is limited homology throughout the 5' flanking sequences with no obvious conserved region. Using an oligonucleotide primer extension assay, specific for the basic PR-1 gene, we have found that this gene is not coordinately expressed with PR-la, b or c.

EMBL accession number: X14065 PRP1.