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Isolation of the gene encoding the major endoglucanase of Erwinia chrysanthemi. Homology between cel genes of two strains of Erwinia chrysanthemi

✍ Scribed by Mari-Hélène Boyer; Brigitte Cami; Alain Kotoujansky; Jean-Pierre Chambost; Chantal Frixon; Jeanne Cattanéo


Book ID
109313864
Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
500 KB
Volume
41
Category
Article
ISSN
0378-1097

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The cya gene region of Erwinia chrysanth
✍ Hedegaard, Lisbeth ;Danchin, Antoine 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 560 KB

A DNA fragment containing the cya gene region ofErwinia chrysanthemi, B374 was cloned in vivo and transferred into cells of E. coli using a plasmid pULB113 derived from RP4 followed by subcloning in vitro into the vector pBR322. The cya gene encodes a 95 kDal protein that complemented E. coli cya mu