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Isolation of the Candida tropicalis gene for P450 lanosterol demethylase and its expression in Saccharomyces cerevisiae

โœ Scribed by Chien Chen; Thomas G. Turi; Dominique Sanglard; John C. Loper


Book ID
115760495
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
427 KB
Volume
146
Category
Article
ISSN
0006-291X

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Candida pelliculosa var. acetaetherius is a strain of yeast which can utilize cellobiose as the carbon source. From a gene library prepared from this yeast, the beta-glucosidase gene has been cloned in a S. cerevisiae host using a chromogenic substrate, 5-bromo-4-chloro-3-indolyl-beta-glucoside as a