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Isolation of Temperature-sensitive Mutants of 16 S rRNA in Escherichia coli

✍ Scribed by Kathleen Triman; Elisa Becker; Carol Dammel; John Katz; Hiro Mori; Stephen Douthwaite; Christos Yapijakis; Sienna Yoast; Harry F. Noller


Book ID
118918832
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
690 KB
Volume
209
Category
Article
ISSN
0022-2836

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✍ Isono, Katsumi ;Krauss, Johanna ;Hirota, Yukinori πŸ“‚ Article πŸ“… 1976 πŸ› Springer 🌐 English βš– 538 KB

The ribosomal proteins of temperature-sensitive mutants of Escherichia coli isolated independently after mutagenesis with nitrosoguanidine were analyzed by two-dimensional gel electrophoresis. Out of 400 mutants analyzed, 60 mutants (15%) showed alterations in a total of 22 different ribosomal prote