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A mutant of Escherichia coli temperature sensitive in the biosynthesis of S-adenosylmethionine

โœ Scribed by Bracha Kimchi; Eliora Z. Ron


Book ID
109313745
Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
392 KB
Volume
43
Category
Article
ISSN
0378-1097

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A temperature sensitive RecA protein of
โœ Hickson, Ian D. ;Gordon, Robert L. ;Tomkinson, Alan E. ;Emmerson, Peter T. ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Springer ๐ŸŒ English โš– 470 KB

The temperature sensitive allele recA200 has been cloned into the multiple copy number plasmid pBR322 and the gene product isolated. The purified RecA200 protein is temperature sensitive in ability to cleave the phage lambda and LexA repressors in vitro and also in ability to promote a successful se