Isolation of suppressor sensitive mutants in the Ai gene of phage λ
✍ Scribed by Cesareni, Gianni ;Nasi, Sergio
- Book ID
- 104726501
- Publisher
- Springer
- Year
- 1976
- Tongue
- English
- Weight
- 442 KB
- Volume
- 149
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
✦ Synopsis
Previous experiments have shown that mutations in the Ai gene can suppress the growth defect of lambda N- phages. Many temperature resistant derivatives of phage lambdatsN9 have been isolated and among these 5 have been found which are Ai- and have an amber suppressible behaviour. These mutants can help in defining the role of the Ai gene in phage lambda development.
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## A suppressor-containing strain of Mycobacterium smegmatis SN 2 was isolated by transferring an amber suppressor carried on the plasmid of Pseudomonaspseudoalcaligenes ERA through transformation. Amber mutants of mycobacteriophage 13 were isolated.