A method to obtain amber mutations in ribosomal protein genes is described. tit relies on the P1-mediated localized mutagenesis (Hong and Ames, 1971) and on the fact that the recipient strain contains (a) an efficient but genetically unstable suppressor, (b) a particular thermoinducible lambda proph
Interfamilial transfer of amber suppressor gene for the isolation of amber mutants of mycobacteriophage I3
β Scribed by T. Ramakrishnan; M. S. Shaila
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 197 KB
- Volume
- 120
- Category
- Article
- ISSN
- 0302-8933
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β¦ Synopsis
A
suppressor-containing strain of Mycobacterium smegmatis SN 2 was isolated by transferring an amber suppressor carried on the plasmid of Pseudomonaspseudoalcaligenes ERA through transformation. Amber mutants of mycobacteriophage 13 were isolated.
π SIMILAR VOLUMES
We describe here the isolation of a mutant derivative of the drug resistance factor R1 (Meynell and Datta, 1966) that carries a nonsense mutation in a gene determining resistance to penicillins. We have used this mutant R1 to isolate derivatives of Escherichia coli and Klebsiella pneumoniae that con
The gene of the amber suppressor tRNA derived from tRNATry, Su+7, has been inserted into a col E1-derived vehicle by selecting for its expression. Despite selection for a suppressor phenotype, and the plasmid's stable presence at ca. 180 copies cell during balanced growth, the level mature tRNA main