Isolation of serum chylomicrons prior to density gradient ultracentrifugation of other serum lipoprotein classes
โ Scribed by A.H.M. Terpstra
- Book ID
- 102984895
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 504 KB
- Volume
- 150
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
A method is presented for the visualization and localization of lipoprotein bands in density gradient ultracentrifugation. The lipoproteins are stained with Sudan black prior to uhracentrifugation. The technique is suitable for studying the lipoprotein patterns of normo-and hyperlipemic sera of both
Starting from a single-spin uhracentrifugation procedure described previously (Foreman et al., J. Lipid Res. 18, 759, 1977), we have improved the system for detection of the fractions eluted from the gradient by monitoring them continuously at 280 nm. A graphic display readily permits assessment of