Isolation of protoplasts from differentEucalyptusspecies and preliminary studies on regeneration

✍ Scribed by C. Teullères; A. M. Boudet

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 860 KB
• Volume: 25
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00042184

✦ Synopsis

Protoplasts were isolated from different Eucalyptus clones and hybrids using mesophyll tissue, calli and cell suspension cultures. The protoplast yields differed greatly according to the starting material and adaptations of the basic procedure had to be designed in specific cases.

Eucalyptus protoplasts are representative of recalcitrant woody plant systems since their proliferation is limited in culture. The best results were obtained with protoplasts from cell suspension cultures. A screening of factors increasing proliferation was performed. When some of these factors were combined the cell division frequency was enhanced and microcalli were obtained.