Isolation of myoblasts from primary mass cultures of embryonicDrosophilacells
β Scribed by Eric Fyrberg; J. James Donady; Sanford Bernstein
- Book ID
- 105006175
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 190 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1573-0603
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Using a subtractive hybridization method, we have cloned 48 cDNAs which are expressed in human primary myoblasts but down-regulated in the embryonal-rhabdomyosarcoma (RMS) cell line RD. Twenty-nine sequences could be identified as coding for previously known gene products, while 19 encode unknown pr
Studies on the effects of time and passage on porcine primary muscle cell cultures and methods to purify myoblasts were conducted using flow cytometry and fluorescence-activated cell sorting (FACS). Primary muscle cells cultured on single plates revealed a small cell (<10 mm diameter) population con