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Isolation and characterisation of oncornavirus from primary cultures of tissues from cattle with leukemia

โœ Scribed by M. I. Parfanovich; N. P. Kasak; A. E. Baranov; T. L. Napastnikova; T. A. Nikolskaya; V. M. Zhdanov

Publisher
Springer Vienna
Year
1977
Tongue
English
Weight
916 KB
Volume
53
Category
Article
ISSN
1432-8798

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โœ Rahman, Z. ;Reedy, A. ;Heatfield, B.M. ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Springer ๐ŸŒ English โš– 597 KB

Trypsinization (WT) was employed to disaggregate urothelial cells from normal human urinary bladder mucosa for the preparation of primary cultures. Urinary bladders of two male adults both 25 years old were obtained autopsy 1-2 h after death. The mucosa was incubated in HBSS containing 0.25% trypsin