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Isolation of Large Oligonucleotide Fragments from the Alanine RNA

โœ Scribed by Jean Apgar, George A. Everett and Robert W. Holley


Book ID
123657763
Publisher
National Academy of Sciences
Year
1965
Tongue
English
Weight
195 KB
Volume
53
Category
Article
ISSN
0027-8424

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An assay for the endonucleolytic cleavag
โœ M.V. Berridge; A.I. Aronson ๐Ÿ“‚ Article ๐Ÿ“… 1973 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 596 KB

A rapid assay for endonuclease activity which cleaves high-molecularweight RNA to acid-precipitable fragments has been developed. RNA is covalently coupled to beaded agarose under conditions that produce relatively few coupling sites. The immobilized RNA can be used qualitatively or semiquantitative