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Isolation of hexose-6-phosphate dehydrogenase from rat liver microsomal fraction by affinity chromatography

✍ Scribed by Yukinobu Hino; Shigeki Minakami


Book ID
118315560
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
447 KB
Volume
98
Category
Article
ISSN
0006-291X

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The isolation of glucose-6-phosphate dehydrogenase from outdated human erythrocytes (type AB+) utilizing NADP+ affinity chromatography and gel-permeation chromatography (Bio-Gel P-150) yields a fully active, homogeneous preparation. The procedure is faster and gives a higher yield than previous repo