Isolation of hepatic microsomes by polyethylene glycol 6000 fractionation of the postmitochondrial fraction

✍ Scribed by Theo A. van der Hoeven

• Book ID: 102984475
• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 527 KB
• Volume: 115
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(81)90024-5

✦ Synopsis

A method is described for the isolation of hepatic microsomes by polyethylene glycol 6000 fractionation of the postmitochondrial fraction of liver homogenate. The procedure is simple and rapid requiring two centrifugation steps at 8000g for IO min. The preparation has peptide patterns and levels of drug metabolic and other enzymatic activity similar to those of the microsomal fraction isolated by high-speed centrifugation and is referred to as polyethylene glycol 6000 microsomes. It is clarified with detergents and can serve as the starting material for the purification of microsomal proteins.