Isolation of full-size mRNA from cells sorted by flow cytometry
β Scribed by Claudius Diez; Gerald Bertsch; Andreas Simm
- Book ID
- 117331553
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 783 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0165-022X
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π SIMILAR VOLUMES
This paper describes an efficient method to concentrate for electron microscopic examination minute quantities of subcellular particles obtained by cytofluorimetric sorting. The advantages of this micromethod, based on diafiltration on Millipore filters under constant positive nitrogen pressure, are
## Abstract ## Background: RNA from sorted cell populations is crucial in many instances. We therefore compared four current protocols for RNA isolation, with regard to mRNA yield and purity. Moreover, we examined the effects on RNA recovery caused by different storage reagents. ## Methods: Smal