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Electron microscopy microsampling of isolated nuclei sorted by flow cytometry

✍ Scribed by Nicoletta Zini; Alessandro Matteucci; Stefano Squarzoni; Angela Galanzi; Riccardo Rizzoli; Stefano Papa

Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 394 KB
Volume: 7
Category: Article
ISSN: 0196-4763
DOI: 10.1002/cyto.990070617

No coin nor oath required. For personal study only.

✦ Synopsis

This paper describes an efficient method to concentrate for electron microscopic examination minute quantities of subcellular particles obtained by cytofluorimetric sorting. The advantages of this micromethod, based on diafiltration on Millipore filters under constant positive nitrogen pressure, are discussed.

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