✦ LIBER ✦

Isolation of DNA from fungal mycelia and sclerotia without use of density gradient ultracentrifugation

✍ Scribed by Susan W. Biel; F.W. Parrish

Book ID: 102986249
Publisher: Elsevier Science
Year: 1986
Tongue: English
Weight: 716 KB
Volume: 154
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(86)90489-6

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✦ Synopsis

A rapid method for extracting total DNA from Aspergillus flavus and Aspergillus parasiticus has been developed. The procedure can be completed in 2 h and yields 200 to 350 micrograms of DNA from 0.5 to 1.0 g wet wt of mycelia and 150 micrograms from 0.5 g of sclerotia. DNA samples had an OD260/OD280 of 1.6 to 1.8. Most of the DNA was at least 50 kb pairs in size and showed little degradation. DNA prepared by this method was used for restriction endonuclease digestion and Southern blotting. A DNA fragment containing the repeat unit of the ribosomal RNA genes of A. flavus has been identified.

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