Isolation of the major subcellular organelles from mouse liver using Nycodenz gradients without the use of an ultracentrifuge
β Scribed by J.M. Graham; T. Ford; D. Rickwood
- Book ID
- 102985127
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 605 KB
- Volume
- 187
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## One-step subcellular fractionation of rat liver tissue using a Nycodenz density gradient prepared by freezing-thawing and two-dimensional sodium dodecyl sulfate electrophoresis profiles of the main fraction of organelles In the present study, we describe a new procedure using freezing-thawing t
An efficient method for the isolation of RNA from cartilage is described. The difficulties in obtaining RNA from cartilage, a tissue of low cell density and high proteoglycan content, were overcome by making several modifications to the guanidine thiocyanate/cesium chloride method of RNA extraction.