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Isolation of cyanobacterial auxotrophs by direct selection of regulatory-mutant phenotypes

✍ Scribed by Geraldine C. Hall; Roy A. Jensen


Book ID
105263507
Publisher
Springer
Year
1981
Tongue
English
Weight
516 KB
Volume
6
Category
Article
ISSN
0343-8651

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A Cephalosoporium acremonium gene library has been constructed using cosmid vector pYcl. After transformation ofE. coli leuB strain JA221 with cosmids isolated from this gene library a cosmid (pCeleuB43) was isolated which complements the leucine auxotrophy of JA221. The same cosmid is also able to