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Isolation of a selective marker fromCephalosporium acremoniumby complementation of an auxotrophic mutant ofE. coli

✍ Scribed by E. Friedlin; J. Nüesch

Book ID
104768185
Publisher
Springer-Verlag
Year
1984
Tongue
English
Weight
513 KB
Volume
8
Category
Article
ISSN
0172-8083

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✦ Synopsis

A Cephalosoporium acremonium gene library has been constructed using cosmid vector pYcl. After transformation ofE. coli leuB strain JA221 with cosmids isolated from this gene library a cosmid (pCeleuB43) was isolated which complements the leucine auxotrophy of JA221. The same cosmid is also able to complement a leu2 strain of S. cerevisiae. The leuB and leu2 genes both code for ~-isopropylmalate dehydrogenase. The complementation of a leuB E. coli" strain and a leu2 S. cerevisiae strain strongly suggests that the analogous gene from C. acremonium has been isolated.

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