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Isolation of an autocrine growth factor from hepatoma HTC-SR cells

✍ Scribed by Peter Ove; Mona L. Coetzee; Philip Scalamogna; Antonio Francavilla; Thomas E. Starzl

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
952 KB
Volume
131
Category
Article
ISSN
0021-9541

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✦ Synopsis

A growth factor has been isolated from HTC-SR rat hepatoma tissue culture cells which specifically stimulates DNA synthesis and cell proliferation of the HTC cells that produce it. The factor can be isolated from HTC cell conditioned medium or from an HTC cell extract. This autocrine factor has been purified W f o l d from a postmicrosomal supernatant by successive steps, involving ethanol precipitation, heating at 8OoC for 10 min, chromatography on a DEAE Bio-Gel A column, and chromatography on a heparin-sepharose affinity column. The major peak of activity eluted from the heparin column migrates as a single band on SDS-PAGE with an apparent M, of 60,000. The factor is resistant to acid, heat, and neuraminidase but sensitive to trypsin, papain, and protease.

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