A rapid method for the isolation and purification of amyloplasts from the endosperm of developing grains of Triticum aestivum L. has been developed. Cellfree amyloplasts were mechanically isolated from plasmolysed tissue, and then purified by low-speed centrifugation through a single layer of Nycode
Isolation of amyloplasts from wheat endosperm
โ Scribed by Gail Entwistle; R. Huw Tyson; Tom ap Rees
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 469 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0031-9422
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The aim of this work was to discover which compound(s) cross the amyloplast envelope to supply the carbon for starch synthesis in grains of Triticum aestivum L. Amyloplasts were isolated, on a continuous gradient of Nycodenz, from lysates of protoplasts of endosperm of developing grains, and then in
The accumulation of amyloplast DNA during endosperm development was studied in two cultivars of spring wheat, Triticum aestivum L. 'Chinese Spring' (CS) and 'Spica', small and relatively larger-grained cultivars, respectively. Endosperms were isolated between 9 and 45 days post anthesis (dpa) and th
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