✦ LIBER ✦

Isolation of amylolytic system ofAspergillus oryzae on DEAE amylum

✍ Scribed by Hanus, J. ;Pašek, A. ;Škachová, H.

Publisher: John Wiley and Sons
Year: 1977
Tongue: English
Weight: 547 KB
Volume: 21
Category: Article
ISSN: 0027-769X
DOI: 10.1002/food.19770210205

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✦ Synopsis

Abstract

Studying isolation technique with DEAE amylum the method was found to be suitable for isolation of α‐amylase. After determination of optimal conditions sorption curves were illustrated, from which the amount of DEAE amylum needed for 100% enzyme sorption from solutions of various activity and origin can be calculated. Preparation obtained shows high temperature stability and can be used in food industry and agriculture or after its elution in pharmacy. At the end of the paper laboratory isolation technique is described.

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Isolation of amylolytic system ofAspergillus oryzae by sorption on DEAHP amylum

✍ Škachová, H. ;Pašek, A. ;Hanus, J. 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 304 KB

## Abstract Conditions of effective sorption of amylolytic enzyme from a solution or from fermentative liquid on DEAHP amylum were studied. Isolating action is in a direct dependence on the relation between activity and amount of DEAHP amylum, the curve of this dependence was illustrated. The enzym