✦ LIBER ✦

Isolation of amylolytic system ofAspergillus oryzae by sorption on DEAHP amylum

✍ Scribed by Škachová, H. ;Pašek, A. ;Hanus, J.

Publisher: John Wiley and Sons
Year: 1977
Tongue: English
Weight: 304 KB
Volume: 21
Category: Article
ISSN: 0027-769X
DOI: 10.1002/food.19770210206

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✦ Synopsis

Abstract

Conditions of effective sorption of amylolytic enzyme from a solution or from fermentative liquid on DEAHP amylum were studied. Isolating action is in a direct dependence on the relation between activity and amount of DEAHP amylum, the curve of this dependence was illustrated. The enzyme can be released by elution or adsorbate can be used in a pulverised form. In the conclusion of the work laboratory isolation technique is described.

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Isolation of amylolytic system ofAspergillus oryzae on DEAE amylum

✍ Hanus, J. ;Pašek, A. ;Škachová, H. 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 547 KB

## Abstract Studying isolation technique with DEAE amylum the method was found to be suitable for isolation of α‐amylase. After determination of optimal conditions sorption curves were illustrated, from which the amount of DEAE amylum needed for 100% enzyme sorption from solutions of various activi