A rapid method for the isolation of DNA from mouse cells grown in tissue culture is described. Cells are lysed in 0.24 M sodium phosphate, pH 6.8 buffer, containing 1% sodium dodecyl sulfate, 8 M urea, and 10m3 M ethylenediaminetetraacetic acid, and the crude lysate applied to a column of hydroxyapa
Isolation of alkaloids of Erythrina from tissue culture
✍ Scribed by R. García-Mateos; M. Soto-Hernández; M. Martínez-Vázquez; A. Villegas-Monter
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 65 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0958-0344
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✦ Synopsis
Seeds of two species of Erythrina (Leguminosae) were examined for the presence of biologically active alkaloids. Undifferentiated callus of Erythrina coralloides and E. americana produced the tetracyclic alkaloids typical for each species, but an unexpected profile of oxygenated alkaloids was observed. The tissue was analysed after isolation and purification, and identification of the alkaloids was by gas chromatography-mass spectrometry (GC-MS). A higher amount of alkaloid was observed in seeds compared with callus of E. americana. A considerable difference is observed when the alkaloid content from callus is compared with that of the intact plant.
📜 SIMILAR VOLUMES
## Abstract isolation and structural elucidation of new Erythrinan alkaloids (I), (II) and (III)
soluble hydrochlorides uncharacterized bases (7 mg.), RI 0.22 and 0.34; Amax. 232 (inflection), 285 nm.; erythraline (21 mg.