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Isolation of a protease-deficient mutant of Bacillus brevis and efficient secretion of a fungal protein disulfide isomerase by the mutant

✍ Scribed by Tsutomu Kajino; Kiyoko Kato; Chie Miyazaki; Osamu Asami; Masana Hirai; Yukio Yamada; Shigezo Udaka

Book ID
114399771
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
219 KB
Volume
87
Category
Article
ISSN
1389-1723

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## Abstract The stabilities of __Bucillus circuluns__ xylanase and a disulfide‐bridge‐containing mutant (S100C/N148C) were investigated by differential scanning calorimetry (DSC) and thermal inactivation kinetics. The thermal denaturation of both proteins was found to be irreversible, and the appar