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Insights into the mechanism and regulation of pyruvate carboxylase by characterisation of a biotin-deficient mutant of the Bacillus thermodenitrificans enzyme

✍ Scribed by Abdussalam Adina-Zada; Sarawut Jitrapakdee; Kathy H. Surinya; Matthew J. McIldowie; Matthew J. Piggott; W. Wallace Cleland; John C. Wallace; Paul V. Attwood


Book ID
116307531
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
315 KB
Volume
40
Category
Article
ISSN
1357-2725

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Insights into the mechanisms of catalysi
✍ Lei Jin; Boguslaw Stec; William N. Lipscomb; Evan R. Kantrowitz πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 529 KB

A high-resolution structure of Escherichia coli aspartate transcarbamoylase has been determined to 2.1 Γ…; resolution in the presence of the bisubstrate analog N-phosphonacetyl-L-aspartate (PALA). The structure was refined to a free R-factor of 23.4% and a working R-factor of 20.3%. The PALA molecule