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Isolation of a new cellulase gene from a thermophilic anaerobe and its expression inEscherichia coli

โœ Scribed by Hiroyuki Honda; Takao Saito; Shinji Iijima; Takeshi Kobayashi

Publisher
Springer
Year
1988
Tongue
English
Weight
386 KB
Volume
29
Category
Article
ISSN
1432-0614

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๐Ÿ“œ SIMILAR VOLUMES

Molecular cloning of a new endoglucanase
Molecular cloning of a new endoglucanase gene fromClostridium cellulolyticumand its expression inEscherichia coli
โœ Seigo Shima; Yasuo Igarashi; Tohru Kodama ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer ๐ŸŒ English โš– 481 KB

Two genes coding for endoglucanase activity in Clostridium cellulolyticum were cloned and expressed in Escherichia coli by using plasmid pUC18. The sizes of two fragments harbouring endoglucanase genes are 4.4 kb and 2.0 kb, respectively. The 2.0-kb fragment was identical with a reported D N A fragm