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Isolation of a human prostate carcinoma cell line (DU 145)

✍ Scribed by Kenneth R. Stone; Don D. Mickey; Heidi Wunderli; George H. Mickey; David F. Paulson

Publisher
John Wiley and Sons
Year
1978
Tongue
French
Weight
943 KB
Volume
21
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

A long‐term tissue culture cell line has been derived from a human prostate adenocarcinoma metastatic to the brain. The cell line, DU 145, has been passaged 90 times in vitro over a period of 2 years. The cells are epithelial, grow in isolated islands on plastic Petri dishes, and form colonies in soft agar suspension culture. Karyotypic analysis demonstrates an aneuploid human karyotype with a modal chromosome number of 64. Distinctive marker chromosomes (a translocation Y chromosome, metacentric minute chromosomes and three large acrocentic chromosomes) have been identified. Electron microscopy of the original tumor tissue and of the tissue culture cell line show a remarkable similarity in cell organelle structure.

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