Identification by C-banding of two human prostate tumour cell lines, 1013L and DU 145

✍ Scribed by Beryl Hartley-Asp; Anita Billström; Elisabeth Kruse

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: French
• Weight: 954 KB
• Volume: 44
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910440128

✦ Synopsis

Two human prostate carcinoma cell lines are easily distinguished by C-banding. DU 145 has one metacentric chromosome with a large centromeric band, I metacentric and 2 acrocentric chromosomes with interstitial C-bands. The Y chromosome was present in 48% and 42% of the cells at passages 83 and 106, respectively, whereas in 1013L only one inetacentric chromosome contained distinct extra C bandsone at the centrornere and one interstitially. The Y chromosome was present in 72% of the cells at passage I5 and in 20% at passage 22, but by passage 44 it had disappeared. Karyotype analysis using G-banding revealed that DU 145 had retained several of its original markers, and demonstrated multiple marker chromosomes in I01 3L.