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Isolation of a genomic DNA forJatropha curcasribosome inactivating protein and its tobacco transformation

✍ Scribed by Yan-yun Luo; Qin Wei; Ming-xing Huang; Ying Xu; Fang Chen

Book ID
107482402
Publisher
Chinese Electronic Periodical Services
Year
2006
Tongue
English
Weight
507 KB
Volume
10
Category
Article
ISSN
1007-6417

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An 11 kb Eco RI genomic fragment containing the alcohol dehydrogenase (Adhl) gene was cloned. Cross-hybridization with three Adh2 cDNA clones suggested that the entire coding region of the Adhl gene was contained on a 6.2 kb Xba I/Hind III subfragment. Using RFLP linkage analysis, the genomic clone