Isolation of a 6.2 kb genomic fragment carrying theAdh1gene of tomato and its expression in transgenic tobacco

✍ Scribed by Ellen Wisman; Marian Bergervoet; Ruud Verkerk; Pim Zabel

• Book ID: 104615283
• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 493 KB
• Volume: 23
• Category: Article
• ISSN: 0167-4412
• DOI: 10.1007/bf00019311

✦ Synopsis

An 11 kb Eco RI genomic fragment containing the alcohol dehydrogenase (Adhl) gene was cloned. Cross-hybridization with three Adh2 cDNA clones suggested that the entire coding region of the Adhl gene was contained on a 6.2 kb Xba I/Hind III subfragment. Using RFLP linkage analysis, the genomic clone was mapped on chromosome 4 between the markers TG 182 and TG 65 in a position corresponding to the Adhl locus. To further confirm the Adhl origin of the genomic clone, tobacco plants were transformed with the 6.2 kb Xba I/Hind III genomic subfragment. Isozyme analysis demonstrated that in transgenic tobacco plants functional tomato specific ADH-1 homodimers were synthesized as well as heterodimers composed of tobacco and tomato subunits.