Rapid isolation of mouse DNA from cells
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William Meinke; David A. Goldstein; Mark R. Hall
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Article
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1974
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Elsevier Science
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English
โ 403 KB
A rapid method for the isolation of DNA from mouse cells grown in tissue culture is described. Cells are lysed in 0.24 M sodium phosphate, pH 6.8 buffer, containing 1% sodium dodecyl sulfate, 8 M urea, and 10m3 M ethylenediaminetetraacetic acid, and the crude lysate applied to a column of hydroxyapa