Isolation of a cDNA from Arabidopsis thaliana that complements the sec14 mutant of yeast
β Scribed by Nathalie Jouannic; Marc Lepetit; Chantal Vergnolle; Catherine Cantrel; Anne-Marie Gardies; Jean-Claude Kader; Vincent Arondel
- Book ID
- 114429495
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 485 KB
- Volume
- 258
- Category
- Article
- ISSN
- 1432-1327
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A yeast null mutant (__erg 3__) defective in __ERG 3__, the gene encoding the Cβ5 sterol desaturase required for ergosterol synthesis was transformed with an __Arabidopsis thaliana__ cDNA library inserted in a yeast vector. Transformants (4Γ10^5^) were screened for cycloheximide (CH) resistance and
Chaperonin (Cpn) is one of the molecular chaperones. Cpn10 is a co-factor of Cpn60, which regulates Cpn60-mediated protein folding. It is known that Cpn10 is located in mitochondria and chloroplasts in plant cells. The Escherichia coli homologue of Cpn10 is called GroES. A cDNA for the Cpn10 homolog
## Summary It is currently thought that most flowering plants lack the capacity to synthesize trehalose, a common disaccharide of bacteria, fungi and invertebrates that appears to play a major role in desiccation tolerance. Attempts have therefore been made to render plants more droughtβresistant b