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Isolation of an Arabidopsis thaliana cDNA by complementation of a yeast abc1 deletion mutant deficient in complex III respiratory activity

✍ Scribed by B. Cardazzo; P. Hamel; W. Sakamoto; H. Wintz; G. Dujardin


Book ID
117523315
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
517 KB
Volume
221
Category
Article
ISSN
0378-1119

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A yeast null mutant (__erg 3__) defective in __ERG 3__, the gene encoding the C‐5 sterol desaturase required for ergosterol synthesis was transformed with an __Arabidopsis thaliana__ cDNA library inserted in a yeast vector. Transformants (4×10^5^) were screened for cycloheximide (CH) resistance and