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Isolation of a cDNA encoding mitochondrial citrate synthase fromArabidopsis thaliana

✍ Scribed by Erica A. Unger; J.Mark Hand; Anthony R. Cashmore; Aurea C. Vasconcelos

Publisher: Springer
Year: 1989
Tongue: English
Weight: 918 KB
Volume: 13
Category: Article
ISSN: 0167-4412
DOI: 10.1007/bf00015553

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✦ Synopsis

We isolated a cDNA clone from Arabidopsis thaliana encoding the TCA cycle enzyme, citrate synthase. The plant enzyme displays 48 ~o and 44~o amino acid residue similarity with the pig, and yeast polypeptides, respectively. Many proteins, including citrate synthase, which are destined to reside in organelles such as mitochondria and chloroplasts, are the products of the nucleocytoplasmic protein synthesizing machinery and are imported post-translationally to the site of function. We present preliminary investigations toward the establishment of an in vitro plant mitochondrial import system allowing for future studies to dissect this process in plants where the cell must differentiate between mitochondria and chloroplast and direct their polypeptides appropriately.

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