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Isolation, immunophenotyping and in vitro functional characterization of murine placenta-derived cells

✍ Scribed by I. Bergwerf; M. Magatti; S. De Munari; S. Acali; D. Rossi; L. Ressel; A. Cargnoni; P. Ponsaerts; O. Parolini


Book ID
113854846
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
51 KB
Volume
32
Category
Article
ISSN
0143-4004

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Abelson murine leukemia virus was used to transform cells of the midgestation embryonic placenta. The frequency of transformed foci in semisolid agarose was highest when cells were isolated at 10 days of gestation and cell lines could be established in liquid culture. The continuous cell lines expre