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Isolation and site-directed mutagenesis of DNA methyltransferaseSssI

โœ Scribed by M. V. Darii; O. V. Kirsanova; V. L. Drutsa; S. N. Kochetkov; E. S. Gromova


Book ID
110174423
Publisher
SP MAIK Nauka/Interperiodica
Year
2007
Tongue
English
Weight
219 KB
Volume
41
Category
Article
ISSN
0026-8933

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## Background A variety of techniques have been described which introduce scarless, site-specific chromosomal mutations. These techniques can be applied to make point mutations or gene deletions as well as insert heterologous DNA into bacterial vectors for vaccine development. Most methods use a mu