Isolation and properties of isocitrate lyase from Lupinus seed
β Scribed by M.T. Vincenzini; F. Nerozzi; F.F. Vincieri; P. Vanni
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 459 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0031-9422
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A NADP+-specific isocitrate dehydrogenase (EC 1.1.1.42) was isolated and purified over 400-fold from Anacystis nidulans. The enzyme activity responded slowly to rapid changes in ligand (NADP +, isocitrate, Mg 2 +-ions) or enzyme concentration as well as to rapid changes in temperature. These are pro
Seed extracts obtained from Lupinus albus and Lupinus angustifolius by treatment with 48% ethanol contained ca. 10% alkaloids (on a dry weight basis) and were non-toxic. Their acute toxicity ( L D ~~) in the mouse is > 4000 mgkg-' body wt. After fractionation, the extract from L. angustifolius seed