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Isolation and primary culture of adult pig hepatocytes

✍ Scribed by X-D. Zhou; T. Tokiwa; J. Kano; M. Kodama

Book ID
110281265
Publisher
Springer
Year
1998
Tongue
English
Weight
136 KB
Volume
19
Category
Article
ISSN
1573-0603

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A technique is described for isolation and culture of adult human hepatocytes from surgical liver biopsies. The mean cell yield was 1.75 X 10(7) cells per gm liver and viability averaged 80%. Hepatocytes were maintained in primary culture for about 10 days. Cell morphology and histochemical characte