Isolation and partial characterization of a “spawning female-specific substance” in the teleost,Oryzias latipes

✍ Scribed by Hamazaki, Tatsuo S. ;Iuchi, Ichiro ;Yamagami, Kenjiro

• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 621 KB
• Volume: 242
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402420313

✦ Synopsis

A "spawning female-specific substance" (SF substance) [Hamazaki et al. (1985) J. Exp. Zool., 235:269-2791 was isolated from the ascites fluid of the estrogen-fed (EF) male or nonspawning female fish, Oryzias latipes, by gel filtration chromatography, followed by high-performance ion-exchange liquid chromatography (HPLC). Biochemical analyses revealed that the SF substance was a glycoprotein that contained 3.01% carbohydrates and showed 49,000 molecular weight (mw) on SDS-PAGE. Amino acid composition and the carbohydrate content of the SF substance were similar to those of the glycoproteins, F1 and F2, which were purified from the hatching enzyme digest of the inner layer (zona internal of egg chorion (egg envelope).

The inner layer of chorion from ovarian egg (oocyte) was separable into three different glycoproteins, ZI-1 (mw 76,0001, ZI-2 (mw 74,0001, and ZI-3 (mw 49,000) on SDS-PAGE. The major glycoprotein, ZI-3, was found to show the mw and amino acid composition that were almost identical to those of the purified SF substance. These results indicate that a substance that is almost the same as the major component glycoprotein of the inner layer of chorion, in terms of the biochemical as well as immunological properties, is present outside the ovary and is produced in the liver of the spawning female fish as well as the EF fish.