A spawning female-specific substance reactive to anti-chorion (egg envelope) glycoprotein antibody in the teleost,Oryzias latipes

✍ Scribed by Hamazaki, Tatsuo ;Iuchi, Ichiro ;Yamagami, Kenjiro

• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: English
• Weight: 880 KB
• Volume: 235
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402350214

✦ Synopsis

A substance with high immunoreactivity to rabbit anti-chorion (egg envelope) glycoprotein antibody was found in liver and blood plasma as well as ovary of the spawning female medaka, while it was absent in the male and the non-spawning female fish. This substance disappeared first from the hepatic cells of the female fish when it ceased spawning, whereas the immunoreactivity still remained in ovary and blood plasma. The relative electrophoretic mobility of the antigenic substance derived from these tissues was identical. The molecular weight and isoelectric point (PI) of the substance, as determined by polyacrylamide electrophoresis with varied gel concentrations and isoelectric focusing method, were about 60,000 and about 4.5, respec-