Isolation and nucleotide sequence determination of a gene encoding a heat-stable enterotoxin of Escherichia coli
β Scribed by Xin Zhou; Li-Ping Shen; Cheng-Wu Chi
- Book ID
- 119007800
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 215 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0041-0101
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
This paper describes the isolation and the purification Of'Escherichia coli heat-stable enterotoxin. The toxin was produced by the E. coli strain Fl 1 (PI 55) of porcine origin. It was purified to homogeneity by successive operations of desalting on XAD-2 resin, gel filtration on Bio-Gel P4, and hig
The hemA gene of Escherichia coli K12 was cloned by complementation of a hemA mutant of this organism. Subcloning of the initial 6.0 kb HindIII fragment allowed the isolation of a 1.5 kb NheI-AvaI fragment which retained the ability to complement the hemA mutant. DNA sequencing by the dideoxy chain