Isolation and purification of Escherichia coli heat-stable enterotoxin of porcine origin

✍ Scribed by R. Lallier; F. Bernard; M. Gendreau; C. Lazure; N.G. Seidah; M. Chrétien; S.A. St-Pierre

• Book ID: 102984598
• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 652 KB
• Volume: 127
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90171-3

✦ Synopsis

This paper describes the isolation and the purification Of'Escherichia coli heat-stable enterotoxin. The toxin was produced by the E. coli strain Fl 1 (PI 55) of porcine origin. It was purified to homogeneity by successive operations of desalting on XAD-2 resin, gel filtration on Bio-Gel P4, and high-pressure liquid chromatography on cyanopropyl and octadecylsilane columns. After chemical characterization by quantitative amino acid analysis and determination of its sequence, the heat-stable enterotoxin was shown to be a monomeric octadecapeptide with the following primary structure: H