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Isolation and mapping of bacterial artificial chromosome (BAC) clone containing telomere-associated sequence

โœ Scribed by Xueqian Gong; Shouyi Chen; Jiming Gong; Feng Liu


Publisher
Science in China Press (SCP)
Year
1998
Tongue
English
Weight
671 KB
Volume
41
Category
Article
ISSN
1674-7305

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Cloning and mapping of telomere-associat
โœ Kilian, Andrzej ;Kleinhofs, Andris ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Springer ๐ŸŒ English โš– 586 KB

We present a novel approach to the efficient cloning of telomere-associated sequences and demonstrate its application to the cloning and mapping of these sequences from barley. The method is a modification of the Vectorette PCR technique and allows specific amplification and cloning of subtelomeric